NGS-verification included
Complex genes/inserts up to 15 kb (max plasmid length of 18 kb)
Standard backbones including:
– pAAV2 (with CMV, CAG, hSYN promoters or ITRs only)
– pUC19
– pBR322
– psaRNA
– pET28
– pcDNA3.1
ENFINIA™ Plasmid DNA brings the power of long, linear, sequence-perfect ENFINIA DNA into plasmid format—delivering high-capacity, NGS-verified DNA up to 18 kb in as fast as 10 business days. Designed for researchers who need both speed and precision, ENFINIA supports applications from synthetic biology to gene therapy development, enabling faster iteration cycles, lower costs, and higher throughput than conventional clonal gene synthesis approaches.
Low-copy, with options for CMV, CAG, hSyn promoters, or ITRs only
Low-copy, includes NS1-NS4 protein sequences
Low-copy, includes Ampicillin resistance gene
High-copy, includes Ampicillin resistance gene
ENFINIA Plasmid DNA can be ordered at the following synthesis yields for high- or low-copy vectors:
1. ENFINIA Plasmid DNA can be ordered at a standard synthesis yield of 50 ng to 1 μg†, depending on the vector selected.
2. ENFINIA Plasmid DNA is delivered as dried-down DNA in a 96-well microplate, one plasmid per well.
3. Each ENFINIA Plasmid DNA sequence is NGS-verified before shipment.
4. Insert sequences should not contain elements that are toxic to E.coli.
* At this time, the QC methods we use for production do not provide high confidence in the fidelity of A/T homopolymer sequences longer than 12 bp
and G/C homopolymer sequences longer than 8 bp. We are working to address this in the near future.
† Synthesis yield as measured by Thermo Fisher Scientific Quant IT Assay.
ENFINIA Plasmid DNA can be ordered at the following synthesis yields for high- or low-copy vectors:
Rapid clonal gene synthesis of longer and more complex genes eliminates the time and effort typically spent subcloning short, error-prone fragments.
Rapidly prototype therapeutic candidates in delivery-compatible vectors for in vitro or preclinical validation.
Streamline clonal gene synthesis to transition from design to functional testing faster.
* Required
Please note that generic email domains (i.e. gmail, yahoo) will not be accepted
