NGS-verification included
Complex genes/inserts up to 15 kb (max plasmid length of 18 kb)
Standard backbones including:
– pAAV2 (with CMV, CAG, hSYN promoters or ITRs only)
– pUC19
– pBR322
– psaRNA
ENFINIA Plasmid DNA is a high-quality, long linear DNA product cloned into a plasmid backbone, offering researchers an unmatched combination of speed, length, and sequencing accuracy. Engineered and sequenced at our U.S.-based facilities, ENFINIA plasmids ship in as fast as 10 business days and are designed to keep up with high-throughput, iterative design cycles common in synthetic biology, gene therapy development, and molecular cloning.
Plasmids can reach up to 18 kb in total length with inserts as long as 15 kb, making ENFINIA ideal for delivering long and complex constructs with confidence. Each clone is verified by NGS to ensure sequence perfection and is predominantly supercoiled for optimal performance in downstream applications. A library of widely used vectors—such as pAAV2 (with CMV, CAG, hSyn, or AAV2 ITRs), pUC19, pBR322, and psaRNA—gives scientists the flexibility to start with a format that meets their expression or delivery needs.
Delivered as dried-down DNA in a 96-well plate format, ENFINIA Plasmid DNA is the right solution for labs that require precision, speed, and scalability in their cloning workflows.
Low-copy, with options for CMV, CAG, hSyn promoters, or ITRs only
Low-copy, includes NS1-NS4 protein sequences
Low-copy, includes Ampicillin resistance gene
High-copy, includes Ampicillin resistance gene
1. ENFINIA Plasmid DNA can be ordered at a standard synthesis yield of 50 ng to 1 μg†, depending on the vector selected.
2. ENFINIA Plasmid DNA is delivered as dried-down DNA in a 96-well microplate, one plasmid per well.
3. Each ENFINIA Plasmid DNA sequence is NGS-verified before shipment.
4. Insert sequences should not contain elements that are toxic to E.coli.
* At this time, the QC methods we use for production do not provide high confidence in the fidelity of A/T homopolymer sequences longer than 12 bp
and G/C homopolymer sequences longer than 8 bp. We are working to address this in the near future.
† Synthesis yield as measured by Thermo Fisher Scientific Quant IT Assay.
Researchers using automation and multiplexed cloning benefit from ENFINIA Plasmid DNA’s rapid turnaround and 96-well plate delivery format. Both enable rapid prototyping of gene circuits and metabolic pathways.
Universities and commercial R&D groups alike can leverage ENFINIA Plasmid DNA’s speed, accuracy, and supercoiled purity to streamline everything, from standard cloning to protein expression and functional assays.
Therapeutic development teams requiring accurate and high-capacity DNA sequences can use ENFINIA Plasmid DNA to rapidly prototype constructs in pAAV2 or other delivery-compatible vectors for in vitro or preclinical validation.
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