ENFINIA™ Plasmid DNA

Plasmids with Genes Up To 15 kb Delivered Fast at an Affordable Price

LONG AND COMPLEX CLONAL GENE SYNTHESIS WITHOUT THE WAIT

NGS-verification included
Complex genes/inserts up to 15 kb (max plasmid length of 18 kb)
Standard backbones including:
– pAAV2 (with CMV, CAG, hSYN promoters or ITRs only)
– pUC19
– pBR322
– psaRNA

 

Up To 15 kb as Fast as 10 Business Days

ENFINIA Plasmid DNA is rapid clonal gene synthesis powered by high-accuracy inserts and standardized vectors to deliver unmatched speed, length, and complexity at an affordable price. With pricing that does not exceed $0.20/bp for insert/gene sequences longer than 3 kb, Elegen now provides long DNA at unprecedented speed and price.

ENFINIA Plasmid DNA Standard Vectors

pAAV2 Vectors

Low-copy, with options for CMV, CAG, hSyn promoters, or ITRs only

psaRNA Vector

Low-copy, includes NS1-NS4 protein sequences

pBR322 Subcloning Vector

Low-copy, includes Ampicillin resistance gene

pUC19 Subcloning Vector

High-copy, includes Ampicillin resistance gene

How ENFINIA Plasmid DNA Meets Your Needs

Sequence Submission Acceptance Criteria

SPECIFICATIONS

1. ENFINIA Plasmid DNA can be ordered at a standard synthesis yield of 50 ng to 1 μg†, depending on the vector selected.
2. ENFINIA Plasmid DNA is delivered as dried-down DNA in a 96-well microplate, one plasmid per well.
3. Each ENFINIA Plasmid DNA sequence is NGS-verified before shipment.
4. Insert sequences should not contain elements that are toxic to E.coli.
* At this time, the QC methods we use for production do not provide high confidence in the fidelity of A/T homopolymer sequences longer than 12 bp
and G/C homopolymer sequences longer than 8 bp. We are working to address this in the near future.
† Synthesis yield as measured by Thermo Fisher Scientific Quant IT Assay.

Literature

ENFINIA™ DNA - A New Class of DNA

ENFINIA™ DNA Cell-free DNA Manufacturing

Brochure

ENFINA Plasmid DNA Specifications and Submission Guidelines

ENFINIA™ Plasmid DNA Specifications & Guidelines

Specs

Plasmid FAQ

FAQ - Ordering ENFINIA™ Plasmid DNA

Document

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