ENFINIA™ Plasmid DNA

LONG AND COMPLEX CLONAL GENE SYNTHESIS WITHOUT THE WAIT

Complex plasmids as large as 19 kb with genes up to 15 kb
Shipped as fast as 10 business days
Growing library of standard backbones

Complex Clonal Gene Synthesis Without The Wait

ENFINIA Plasmid DNA delivers rapid clonal gene synthesis with unmatched speed, length, and complexity at an affordable price. This next-gen combination of capability and affordability can save researchers weeks compared to conventional clonal gene synthesis services, while paying less!

ENFINIA Plasmid DNA Standard Vectors

How ENFINIA Plasmid DNA Meets Your Needs

Sequence Submission Acceptance Criteria

SPECIFICATIONS

1. ENFINIA Plasmid DNA typically ships at yields of: Mini-prep: 50 ng to 1 µg, Midi-prep: 10 µg to 100 µg, Maxi-prep: 100 µg to 1 mg per clone (dependent on sequence and vector). Abnormally low yields are considered a failed synthesis.

2. ENFINIA Plasmid DNA is delivered as dried-down DNA in a 96-well microplate, one plasmid per well.

3. Each ENFINIA Plasmid DNA sequence is NGS-verified before shipment.

4. Insert sequences should not contain elements that are toxic to E.coli.

* At this time, the QC methods we use for production do not provide high confidence in the fidelity of A/T homopolymer sequences longer than 12 bp and G/C homopolymer sequences longer than 8 bp. We are working to address this in the near future.

Midi-prep and Maxi-prep DNA is purified using Macherey-Nagel NucleoBond Xtra Plus EF transfection grade kits and reported as measured by NanoDrop. Mini-prep yields are reported as measured by Thermo Fisher Scientific Quant IT assay.  

Genome Engineering

Rapid clonal gene synthesis of longer and more complex genes eliminates the time and effort typically spent subcloning short, error-prone fragments.

Gene Therapy Vector Construction

Rapidly prototype therapeutic candidates in delivery-compatible vectors for in vitro or preclinical validation.

Protein and Antibody Engineering

Streamline clonal gene synthesis to transition from design to functional testing faster.

Literature

ENFINIA™ DNA - A New Class of DNA

ENFINIA DNA Cell-free DNA Manufacturing

Brochure

ENFINIA Plasmid DNA Specifications & Guidelines

Specs

Plasmid FAQ

FAQ - ENFINIA Plasmid DNA

Document

Want to learn more about ENFINIA Plasmid DNA?

* Required
Please note that generic email domains (i.e. gmail, yahoo) will not be accepted