Topics Covered include:

• Historical perspective on gene synthesis and the evolution of the field from early DNA discoveries to the latest advancements in next generation synthesis
• The limitations of traditional cell-based cloning methods, such as the lengthy and error-prone process of assembling and cloning short DNA fragments.
• Analysis of the trade-offs between in-house DNA construction and outsourcing, in terms of speed, cost, and quality for various applications.
• Benefits of cell-free gene synthesis for various research applications, particularly those involving complex DNA constructs in IVT-mediated mRNA synthesis and AAV delivery systems for genetic medicines.