Application Note – Rapid Cell-Free Protein Expression with ENFINIA™ Linear DNA

Application Note: Rapid Cell-Free Protein Expression with ENFINIA™ Linear DNA

Elegen

Here we demonstrate that ENFINIA Linear DNA templates, synthesized by Elegen, are functional in cell-free TXTL systems and drive reporter gene expression at levels comparable to plasmid DNA templates.

Cell-free transcription-translation (TXTL) systems are used for rapid and flexible protein expression. Traditionally, plasmid DNA has been used as the template for protein expression in cell-free systems, but this requires time-consuming cloning steps.

ENFINIA™ Linear DNA templates, synthesized by Elegen, offer a way to skip the cloning process, streamlining protein expression workflows and saving 1 to 2 weeks compared to in vivo methods.

This application note demonstrates that ENFINIA Linear DNA performs comparably to plasmid DNA in the myTXTL Pro Cell-Free Expression Kit from

If you want to streamline your protein expression workflows, reduce costs, and accelerate your research, download this application note for valuable information and practical insights into the use of ENFINIA Linear DNA templates.