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GEN II Oligonucleotide Synthesis

Automated, miniaturized, cell-free DNA production

High Throughput Microfluidic Oligonucleotide Synthesis

The microfluidic chip miniaturizes the entire oligo synthesis workflow, reducing instrument footprint while dramatically increasing throughput.

Compared with conventional DNA synthesizers, it uses far smaller reagent volumes—lowering operating costs and waste without sacrificing quality or throughput. The result is highly parallel, highly diverse oligo synthesis delivered with exceptional efficiency and scale.

Proprietary steering methodology enables high-throughput precision bead control.
Proprietary steering methodology enables high-throughput precision bead control.
Microfluidic chip can efficiently build a diverse array of oligos in parallel.
Microfluidic chip can efficiently build a diverse array of oligos in parallel.
The oligosynthesizer chip utilizes microfluidics to shuttle beads to different columns where nucleotides are added.
The oligosynthesizer chip utilizes microfluidics to shuttle beads to different columns where nucleotides are added.

HOW IT WORKS

Our oligo synthesizer uses a proprietary microfluidic chip to simultaneously produce thousands of unique single-stranded DNA oligos using a split-pool synthesis approach. Integrated positional encoding enables precise tracking of each oligo for continuous quality control throughout the process.

 

At the end of a synthesis run, oligos are sorted by customer order and dispensed into one or more wells of a microplate.

INSIDE THE CHIP

Using a split-pool synthesis methodology, thousands of oligos are produced through a rapid, cyclical process of nucleotide addition. In each cycle, different groups of oligos receive different nucleotides, enabling the simultaneous construction of thousands of unique sequences in a single run.

 

Every nucleotide addition is quality-controlled to ensure sequence-perfect oligos. Once synthesis is complete, oligos are grouped by order and dispensed into one or more wells of a microplate.

WHY IT MATTERS

Fast, accurate, and versatile—Elegen’s oligo synthesizer produces the building blocks for all ENFINIA DNA products.

 

  • Compact & Efficient: Fits in just 56 sq ft and makes up to 40,000 unique oligos per week.
  • Flexible: Subsetting lets multiple orders run simultaneously, speeding up delivery.
  • End-to-End Power: The foundation of the GEN II platform, which combines AI, robotics, and cell-free cloning to economically and efficiently produce dsDNA.
  • Cost-Effective: Advanced technology reduces waste and production costs, passing savings directly to researchers.

 

The result? High-quality DNA, faster iteration, and quicker access to critical insights.