Learn more about Elegen’s superior ability to produce high-complexity DNA sequences that conventional methods struggle with. Our novel assembly method minimizes hybridization errors during polymerase chain assembly (PCA), enabling the successful synthesis of sequences rich in GC-rich elements, long repeats, and other challenging structures that are frequently rejected by competitors, including:

• Internal repeats (20-300 bp)
• Low GC regions (16-8%)
• High GC regions (80-85%)
• Hairpins (AT 20-50, GC 10-15)
• STRs (9-25 bp)
• Common plasmid parts
• Sequences rejected by previous ENFINIA Acceptance Criteria
• Homopolymers (AT 20-50, GC 10-15)
• GC variable regions
• Terminal repeats (80, 140 bp)

Internal testing demonstrates significantly improved synthesis success rates (92%) compared to leading competitors (≤58%), validating ENFINIA’s ability to handle previously unsynthesizable sequences, such as those found in antibody expression constructs and various promoters.