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High-Efficacy Synthesis with NEB's HiScribe T7 mRNA Kit

The application note demonstrates that Elegen's ENFINIA IVT Ready DNA is a high-quality, efficient, and cost-effective alternative to plasmid-derived DNA for mRNA synthesis using NEB's HiScribe T7 mRNA Kit, yielding equivalent or higher quality mRNA.

mRNA synthesis can be streamlined with ENFINIA™ IVT Ready DNA, a cell-free linear template that eliminates the bottlenecks of traditional plasmid-based workflows.

This application note demonstrates the direct use of Elegen’s NGS-verified, poly(A)-encoded templates with NEB’s HiScribe® T7 mRNA Kit and CleanCap® Reagent AG.

This seamless integration facilitates high-quality mRNA synthesis with co-transcriptional capping to generate a natural Cap-1 structure—all without the need for cloning, purification, or linearization.

Comparative results show that ENFINIA templates yield mRNA with equivalent or superior quality and functionality to linearized plasmids, providing a more efficient, cost-effective path from sequence design to functional lead.