Highly Complex DNA Without Compromise

ENFINIA High-Complexity DNA Synthesis – Now Available
► Get 15% off your next order


Linear DNA from 1 to 7kb – NGS verified
Error rates as low as 1:70,000 per bp
High-complexity DNA shipped in 6 to 8 business days

Now Accepting High Complexity Sequences

Elegen’s latest ENFINIA™ DNA synthesis enhancement provides access to very challenging sequences, including long repeats and STRs, GC-rich promoters, enhancers, terminators, ITRs, LTRs, and homopolymers. Elegen’s cell free process eliminated the need for time-consuming cloning and purification.

figure very complex DNA synthesis

Elegen’s expanded sequence acceptance criteria now enable the synthesis of long linear dsDNA that includes:

  • Inverted terminal repeats (ITRs), including AAV ITRs
  • Difficult promoters and enhancers (e.g. R6K, CaMV 35S, SV40, IRES, GAL4-UAS)
  • Hairpins up to 100bp
  • A/T homopolymers up to 30bp
  • G/C homopolymers up to 15bp
  • Short tandem repeats (STRs) up to 60b
  • Long repeats up to 150bp, including lentivirus LTRs
  • 100bp regions of 12% to 85% GC content
  • Greater flexibility on the position of complex regions



  1. ENFINIA DNA can be ordered at a standard synthesis yield of 1-3 µg or higher synthesis yield of 5-15 µg or 20-60 µg.
  2. ENFINIA DNAwill be delivered as dried down linear double-stranded DNA in a 96 well plate, one sequence per well.
  3. Each ENFINIA DNA sequence contains a 46 bp adapter on the 5’ end and a 42 bp adapter on the 3’ end of the desired sequence. Adapter sequences are provided.
  4. Each ENFINIA DNA sequence is verified by NGS before shipment.

* At this time, the QC methods we use for production do not provide high confidence in fidelity of A/T homopolymer sequences longer than 10bp. A/T homopolymer sequences up to 20bp will be accepted, but we cannot guarantee synthesis quality above 10bp. We are working to address this in the near future.
† Certain sequence complexities or combinations of complexities may not be compatible at lengths greater than 5,500 bp.


15% off your next order of HIGH-COMPLEXITY ENFINA DNA
Valid March 18, 2024, though May 31, 2024

Complete this form to receive an email containing your promo code. Please whitelist emails from elegenbio.com.
Please note that generic email domains (i.e. gmail, yahoo) will not be accepted.