Cell and Gene Therapy and Genome Editing

High-fidelity DNA in versatile formats for advanced therapy development

Don’t let DNA synthesis be your bottleneck.

ENFINIA DNA for Cell and Gene Therapies

ENFINIA DNA offers unparalleled versatility for cell and gene therapy development with industry-leading speed, NGS-verified fidelity, reliability, and affordability in both linear and plasmid formats. The platform’s ability to produce high-complexity sequences allows for the construction of safe, tailored, and more effective personalized therapeutic payloads without compromising on speed, quality, or cost.

This diverse portfolio supports a wide range of applications—from cell-based immunotherapies to in vivo gene editing—ensuring you have the optimal DNA format to drive advances in therapeutics.

IN VIVO CAR-T THERAPY

CAR-T therapies are transforming cancer treatment. New in vivo approaches build therapeutic T cells directly in the patient by delivering CAR-encoding mRNA with targeted nanoparticles—promising faster, more accessible therapies.

How Elegen Helps

Streamlined mRNA Construction
ENFINIA IVT Ready DNA templates are delivered with encoded poly(A) tails, requiring no additional downstream processing. This allows for the rapid construction of CAR-encoding mRNA, enabling researchers to begin testing therapeutic potential without the delays of plasmid cloning.

TCR THERAPY

T-cell receptor (TCR) therapies engineer T cells to target cancer-specific neoantigens. The process moves from mining a patient’s immune repertoire to high-throughput screening, narrowing thousands of ⍶ / β pairs down to the most promising candidates.

From your list of top hits, our synthetic DNA templates enable rapid optimization and validation, ensuring maximum potency and specificity both in vitro and in vivo.

How Elegen Helps

Fast DNA for Validation
ENFINIA Linear DNA streamlines the transition from lead discovery to functional characterization by providing NGS-verified, high-complexity fragments ready for immediate use. By supporting challenging sequences that often stall traditional cloning workflows, ENFINIA ensures your TCR sequences are accurate, high-fidelity, and delivered on a condensed timeline to keep your research moving forward.

Direct-to-IVT Solution
ENFINIA IVT Ready DNA is transcription-ready with poly(A) tail included for rapid, high-quality mRNA production. For in vivo TCR therapies, ENFINIA templates enable the direct delivery of TCR-encoding mRNA into a patient’s T cells, allowing their immune system to generate therapeutic T cells in situ.

ENFINIA DNA for Genome Editing

CRISPR-Cas9 has revolutionized genome editing, enabling a new generation of innovative ex vivo and in vivo therapies. By leveraging cell-free, high-fidelity ENFINIA DNA, researchers can significantly accelerate CRISPR workflows while reducing technical complexity.

VECTOR-MEDIATED DELIVERY

Plasmids are foundational tools for genome editing, providing a versatile means to deliver genetic instructions to target cells. In non-viral approaches, “naked” plasmid DNA can be introduced directly into cells for transient or stable expression of editing machinery.

Beyond direct delivery, plasmids are essential components for producing viral vectors. For instance, adeno-associated virus (AAV) is frequently used for in vivo gene editing, while lentivirus is utilized to stably integrate genome editing machinery into the host genome.

How Elegen Helps

Fast, accurate, plasmid solutions

ENFINIA Plasmid DNA offers the versatility and speed required to advance your genome editing. With a wide selection of vectors, a generous capacity of up to 15 kb, and quick turnaround times, you can rapidly iterate and refine your editing constructs without the traditional cloning logjam.

For viral delivery, transfer plasmids carrying a nuclease (such as Cas9), a guide RNA (gRNA), or both are critical components in the production of engineered viral particles. For AAV-mediated delivery, our pAAV2 backbone provides a reliable foundation for robust in vivo gene editing. We also offer a lentiviral plasmid (pLV-EF1a) for ex vivo editing applications requiring stable genomic integration

In vivo genome editing can be achieved by co-transfecting cells with Cas9 encoded as mRNA alongside a guide RNA within a delivery vehicle like lipid nanoparticles. This approach allows transient genome editing directly within the patient or model.

How Elegen Helps

Transcription-Ready mRNA Templates

ENFINIA IVT-Ready DNA can encode diverse nucleases—including Cas9, base editors, or gene writers—for in vivo applications. Supporting payloads up to 5.5 kb and offering multiple poly(A) tail options, these templates are transcription-ready for immediate, high-efficiency mRNA production.

HDR-Mediated Knock-ins

CRISPR-Cas9 is frequently used to perform HDR-mediated knock-ins, an approach that requires a donor DNA repair template containing the desired sequence flanked by homology arms.

How Elegen Helps

High-performance repair templates

ENFINIA Linear DNA provides a clean, rapid solution for researchers requiring sequence-verified dsDNA templates up to 7 kb. Because it is produced cell-free, it is entirely free of bacterial elements, reducing potential cellular toxicity.

For projects involving larger knock-ins, ENFINIA Plasmid DNA offers a classic, high-capacity solution with industry-leading speed, shipping in as few as 10 business days.

The Elegen Advantage: Accelerate the Design-to-Refine Cycle

Fast Optimization for Better Therapies. Whether you are maximizing CAR T-cell efficacy, minimizing off-target CRISPR effects, or tailoring payloads for novel delivery vehicles, success depends on how fast you can iterate. The traditional path from digital design to physical often depends on DNA synthesis. Unfortunately, most vendors become a bottleneck—requiring weeks for complex sequences or forcing you into slow, manual cloning workflows.

At Elegen, we’ve broken the bottleneck. We deliver long, complex, NGS-verified DNA in as few as 6 business days, so you can proceed immediately with your research. We provide the speed and fidelity needed to power rapid design-test-refine cycles. Our platform supports the industry’s most demanding payloads, delivering reaction-ready dsDNA that fits seamlessly into your therapeutic workflows: