Conventional protein expression workflows are limited by the rate at which cells grow
Conventional protein expression workflows are limited by the rate at which cells grow. Eliminate this bottleneck with cell-free ENFINIA Linear DNA and cell-free protein expression.
STEP ONE
DAYS ⇒ WEEKS
Target Identification
Protein expression starts with selecting a target and optimizing its sequence. Factors such as source organism, localization, secretion signals, modifications, purification, and application influence ORF design. Complex proteins, such as membrane or multi-subunit types, require testing variants with different codons, domains, or chaperones, which increases the demand for fast-turnaround, complex DNA.
STEP TWO
DAYS ⇒ WEEKS
Expression System Selection
The chosen expression system—whether in vivo or in vitro—shapes construct design and DNA format. In vivo systems offer scalable expression with modification capabilities, while in vitro systems are cell-free and provide faster turnaround. Yet, generating synthetic DNA templates for either system typically involves lengthy and labor-intensive cloning.
STEP THREE
2 ⇒ 6 WEEKS
Construct Design and Synthesis
Once an expression system is chosen, ORFs are digitally assembled with regulatory elements and tags for expression, localization, or purification. Codon optimization depends on the host, and constructs may need redesign for different systems. Progress often stalls when suppliers reject sequences for length or complexity, and plasmid generation—internal or outsourced—adds further delay.
How Elegen Helps
Long, complex ENFINIA Linear DNA provides a fast and predictable alternative to classic plasmid-based gene synthesis.
STEP FOUR
1 ⇒ 10 DAYS
Protein Expression
Constructs are introduced into the system, and conditions are optimized for yield, solubility, and activity. In vivo setups require tuning induction, temperature, and media, while in vitro systems enable same-day expression by adding linear or plasmid DNA directly. DNA template quality is crucial for achieving consistent yields, particularly in high-throughput or highly sensitive assays.
How Elegen Helps
ENFINIA Linear DNA is NGS-verified for sequence accuracy and purity before shipment.
STEP FIVE
MONTHS
Refine and Repeat
Additional construct designs are often tested to improve yield, solubility, and activity through iterative refinements. This may involve redesigning the ORF, altering regulatory elements, or trying different tags or fusion proteins. Each cycle requires new DNA.
How Elegen Helps
ENFINIA Linear DNA eliminates the cloning steps associated with plasmid templates, eliminating delays and saving time.
Give Cell-Free Protein Expression a Boost!
Cell-free ENFINIA Linear DNA templates simplify and accelerate cell-free transcription-translation (TXTL) protein expression systems.
At Elegen, we’ve broken the bottleneck in iterative design for protein expression, delivering long, complex, NGS-verified cell-free synthetic DNA in a matter of days, eliminating the delays associated with cloning.
DNA Delivered in days, not weeks.
High complexity sequences welcome
Cloning-free, expression-ready format
Reaction-ready format for faster in vitro transcription and testing
Accelerate and simplify protein expression workflows without compromising on DNA accuracy. No cloning required.
Clonal gene synthesis that won’t slow you down. Long and complex expression constructs are welcome.
